Serum Free CMRL Chemically Defined Medium With Horse Or Calf Serum

CMRL medium for serum-free culture of mammalian cell lines supplemented with horse serum or calf serum

product description:

CMRL medium was designed by Connaught Medical Research Laboratories

Product Usage:

Originally used for serum-free culture of L cells (Earle). After adding horse serum or calf serum, the CMRL medium is especially suitable for the clone culture of monkey kidney cells and the culture of other mammalian cell lines.

This product contains amino acids, vitamins, inorganic salts, and other ingredients required for various types of cell culture, but does not contain proteins, lipids, or any growth factors, so this product should be used with serum or serum-free supplements.

Product ingredients:

NEAA (non-essential amino acids)

On the basis of MEM medium, add L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and Glycine contains 7 kinds of NEAA, which can reduce the side effects of non-essential amino acids produced by cells themselves during cell culture, and effectively promote cell proliferation and metabolism.

Phenol red

It is used as an indicator of pH value in the culture medium to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values, it makes the culture medium purple. , when the pH value is 7.2 to 7.4, it is red, which is most suitable for cell culture. However, phenol red also has some disadvantages. Therefore, it is best to use a medium without phenol red when using it. Phenol red interferes with detection during flow cytometry analysis. In addition, the presence of phenol red in some formulations of serum-free media interferes with sodium-potassium balance.

Penicillin-streptomycin mixed solution is usually also called "double antibody", which is the most commonly used antibiotic to prevent microbial contamination in vitro culture. Among them, penicillin can interfere with the synthesis of bacterial cell walls and is particularly effective against Gram-positive bacteria; while streptomycin Mycin can bind to the 30S subunit of bacterial ribosomes and inhibit the synthesis of bacterial proteins. It is effective against both Gram-negative bacteria and Gram-positive bacteria but is particularly effective against Gram-negative bacteria. The combination of penicillin and streptomycin can prevent most bacterial contamination.

Five advantages of iclean cell culture medium:

1. Complete products, 280 kinds of basic culture media

2. More than 200 kinds of special media with missing components or additional components are available in stock

3. The medium can be customized according to the needs, and the composition/concentration/ph value can be customized, etc.

4. The performance of the product is stable, and it is produced by an automatic filling line, and the difference between product batches is small

5. Strict quality control system, sterility testing, mycoplasma, endotoxin testing, cell growth experiment, light inspection, etc.

Huachenyang Technology Co., Ltd. was established on June 2, 2008. The company has 3 major business segments. The business operation center is located in Yifang Center, Baoan District, Shenzhen, the R&D experiment center is located in Nanshan District, Shenzhen, and the manufacturing center is located in Baoan, Shenzhen. Xinqiao Street, District, the company covers an area of ​​15,000 square meters, with a registered capital of 50 million yuan and more than 300 employees. A national high-tech enterprise integrating R&D, production, and sales of medical device products.

Huachenyang Technology is committed to biological sample collection and preservation of medical products. It is a well-known professional manufacturer of medical sampling and preservation products. The company has 52 patents, of which 30 patents have been transformed into the market. Products are mainly used in genetic testing, biopharmaceuticals, large-scale A-level hospitals, entry-exit inspection and quarantine, diagnostic reagents, CDC, public security criminal investigation, forensic identification, and other medical institutions. The company has successively established good cooperative relations with more than 30 domestic universities, more than 50 national scientific research institutions, more than 200 tertiary hospitals, more than 600 disease prevention and control centers, and more than 1,000 third-party medical testing institutions for genetic testing.

The company's independent research and development products include disposable virus sampling tubes, disposable sampling swabs, medical cotton swabs, cell preservation solutions, saliva collection devices, transport medium, disposable samplers, disposable sampler, sample preservation solution, Nucleic acid extraction reagents, genetic testing reagents, and other products. The company's products have obtained China NMPA, EU CE, US FDA EUA, SGS, TUV, TGA, and ISO13485 certification qualifications, and have the exclusive right to use registered trademarks and sales rights for multinational products. The products are exported to Europe, America, Asia, the Middle East, and other 120 countries. It has won unanimous praise and recognition from customers.

Shenzhen Huachenyang Technology Co., Ltd. will adhere to the tenet of "inheriting innovation, winning with science and technology", adhering to the corporate culture of "quality first, truth-seeking and innovation, integrity and law-abiding, cooperation and win-win", and strive to create China's high-end biological sample collection and preservation medical products, casting The industry benchmark for microbial sample collection and preservation products!